Study on effect of Oviductus Ranae on the expression of p16, p21 and cyclinD1 in liver tissues of aged female rats
- Author:
Hui YAO
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Aging;
Cell cycle;
D-galactose;
Liver;
Oviductus Ranae
- From:
Chinese Pharmaceutical Journal
2012;47(16):1297-1301
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the effect of Oviductus Ranae (OR) on the expression of p16, p21 and cyclinD1 protein in liver tissue of D-galactose-induced aging female rats and to further explore the anti-aging mechanism of OR in female rats. METHODS: Fourty young female SD rats (SPF grade) were randomly divided into 5 groups including OR-H group, OR-M group, OR-L group, VE group, and D-gal group with 8 in each group. The 40 rats received subcutaneous injection of D-galactose on the nape for 42d to establish aging model. Another 8 rats were injected daily with normal saline (NS) at the same position as the normal control group. From the 15th day of the experiment, the rats were given oral OR (in OR group) or Vitamin E (in VE group) for 28 d. Then all the rats were sacrificed. P16 and p21 in liver tissue were detected by immuno-histochemistry (IHC), and cyclinD1 was detected by Western-blotting method. RESULTS: The result of IHC showed that p16 and p21 mainly multifocally located in endochylema of liver cells and their distribution was diffuse and focus. Positive cell integral of p16 and p21 in the liver tissue of D-gal female aged rats was significantly increased than in normal control group (P < 0.01). After taking OR, the integral of p16-positive cell in OR-M and OR-L groups was significantly decreased than in D-gal group (P < 0.01). The integral of p21-positive cell in OR-H, OR-M and OR-L groups was significantly decreased than D-gal group (P < 0.01). The result of Western-blotting showed that the expression of cyclinD1 protein in the liver tissue of D-gal female rats was significantly decreased than in normal control group (P < 0.01). The expression of cyclinD1 in all OR groups was significantly increased compared with D-gal group (P < 0.01). The expression of cyclinD1 in OR-H group was the highest in all OR groups. CONCLUSION: OR can decrease the over-expression of p16 and p21 protein in liver tissue. Meanwhile, OR can enhance the expression of cyclinD1 in the liver of female rats. OR can promote liver cell proliferation of aging female rats. The anti-aging effects of OR is possibly produced through regulation of p16, p21, and cyclinD1 signaling pathways to promote the expression of the proliferation regulatory protein, and the signaling pathways play an important role in delaying aging. Copyright 2012 by the Chinese Pharmaceutical Association.
