Simultaneous determination of the contents and fingerprint of multi-components in honeysuckle extract by HPLC
- Author:
Bing HE
1
Author Information
1. Research Center for Drug
- Publication Type:Journal Article
- Keywords:
Caffeic acid;
Chlorogenic acid;
Cryptochlorogenic acid;
Fingerprint;
Honeysuckle extract;
HPLC;
Isochlorogenic acid A;
Isochlorogenic acid B;
Isochlorogenic acid C;
Neochlorogenic acid
- From:
Chinese Pharmaceutical Journal
2012;47(16):1280-1284
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a HPLC method for the simultaneous determination of the contents and fingerprint of 7 active constituents (neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid B, isochlorogenic acid A, and isochlorogenic acid C) in honeysuckle extract. METHODS: The chromatographic separation was achieved on a AkzoNobel Kromasil C18 column(4.6 mm × 250 mm, 5 μm), the mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution with gradient elution at a flow rate of 1.0 mL · min-1, the detection wavelength was set at 326 nm, and the column temperature was 30°C. RESULTS: All the 7 compounds showed good linearity in the ranges of the test concentrations. The RSDs of the precision, stability and reproducibility tests were less than 3%. The average recoveries were in the range of 97.98%-99.29%. CONCLUSION: This method is simple, sensitive, accurate, and can be used for quality control of honeysuckle extract. Copyright 2012 by the Chinese Pharmaceutical Association.
