Effects of Dan'e Fukang decocted extract on the expression of MMP-9 and TIMP-1 mRNA in endometriosis rats
- Author:
Ke HE
1
Author Information
1. The Forth Hospital of Shijiazhuang
- Publication Type:Journal Article
- Keywords:
Dan'e Fukang decocted extract;
Endometriosis;
Matrix metalloproteinases-9;
Tissue inhibitor of metalloproteinase-1
- From:
Chinese Pharmaceutical Journal
2012;47(17):1381-1384
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the differences of MMP-9 and TIMP-1 mRNA expression between eutopic and ectopic endometrium tissues in endometriosis (EMS) rats, and to observe the effects of Dan'e Fukang decocted extract (DFD) on the expression levels of MMP-9 and TIMP-1 mRNA. METHODS: The EMS rat model was made by autotransplantation and verified by ELISA method. Then the rats were randomly divided into 5 groups, sham operation group, EMS model group, Gestrinone group, high dose DFD group, and low dose DFD group. After continuous intragastric administration for 30 d, the expression levels of MMP-9 and TIMP-1 mRNA were detected by RT-PCR. RESULTS: DFD could significantly down-regulate the expression levels of MMP-9 mRNA in EMS rats (P < 0.01), and significantly up-regulate the expression level of TIMP-1 mRNA (P < 0.01). CONCLUSION: DFD can treat EMS through adjusting the balance of MMP-9 and TIMP-1. Copyright 2012 by the Chinese Pharmaceutical Association.
