Extraction and isolation of Peristrophe in Peristrophe roxburghiana Brem and its content determination
- Author:
Xiao-Qing YAO
1
Author Information
1. School of Pharmaceutical Science and Technology
- Publication Type:Journal Article
- Keywords:
Content determination;
Isolation;
Peristrophe roxburghiana Brem;
Peristrophine
- From:
Chinese Pharmaceutical Journal
2012;47(19):1535-1538
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To extract and isolate peristrophine from Peristrophe roxburghiana Brem and establish a content determination method of peristrophine. METHODS: Whole plant of Peristrophe roxburghiana Brem was extracted with 60% ethanol, then purified with D101 resin and preparative high-performance liquid chromatography (HPLC). High performance liquid chromatography (HPLC) using a mobile phase consisting of 55% methanol was used for the content determination (γ = 236 nm). RESULTS: The lineal range of peristrophine was 5.0 - 50.0 mg · L-1 (r = 0.9998), the mean recovery was 97.67%, and RSD was 0.66% (n = 9). CONCLUSION: This method can be used as the basis for the further research of Peristrophe roxburghiana Brem. Copyright 2012 by the Chinese Pharmaceutical Association.
