Simultaneous determination of flavones in Shuanghuanglian preparations by both QAMS and external standard method
- Author:
Wei-Hong FENG
1
Author Information
1. Institute of Chinese Materia Medica
- Publication Type:Journal Article
- Keywords:
External standard method (ESM);
Flavone;
HPLC;
Quantitative analysis of multi-components by single-marker (QAMS);
Relative correlation factor(RCF);
Shuanghuanglian preparation
- From:
Chinese Pharmaceutical Journal
2012;47(20):1665-1669
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the feasibility of quantitative analysis of multi-components by single-marker (QAMS) in determination of flavones in Shuanghuanglian preparations by comparing the results assayed by QAMS and external standard method (ESM). METHODS: The analysis was performed on a Xtimate™ C18 column (4.6 mm × 250 mm, 5 μm)with methanol and water containing 0.2% phosphoric acid as the mobile phase in gradient elution. The flow rate was set at 1.0 mL · min-1. The UV detector wavelength was 274 nm, and the column temperature was 30°C. RESULTS: Within the linear ranges, the analysis results showed no significant difference between QAMS and ESM. CONCLUSION: The QAMS method is credible and will be a new quality evaluation pattern for the determination of multiple flavones in Shuanghuanglian preparations.
