Quantitative determination of adenovirus and carmustine in anionic liposomes-mediated co-delivery system
- Author:
Zhi-Rong ZHONG
1
Author Information
1. Luzhou Medical College
- Publication Type:Journal Article
- Keywords:
Adenovirus;
Anionic liposome;
Carmustin;
Co-delivery;
HPLC;
Q-PCR
- From:
Chinese Pharmaceutical Journal
2012;47(20):1634-1637
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish effective and reliable methods for the determination of adenovirus and carmustine in anionic liposomes-mediated co-delivery system. METHODS: The complexes of co-delivery system were prepared by calcium-induced phase changing method. For carrying out the quantitative-PCR (Q-PCR) detection, the sequences of primer and probe were designed accoding to the hexon gene sequences of Ad5, and then the reaction system of Q-PCR was optimized to detect the loading rate of Ad5. The HPLC condition was also optimized to determine the drug loading of carmustine in the co-delivery system. RESULTS: In the co-delivery system, the loading rate of adenovirus detected by Q-PCR method was (23.2±1.8)% and that of carmustine was (55±2.8)%. CONCLUSION Both of Q-PCR and HPLC methods have been successfully used for the quantification of adenovirus and carmustine in anionic liposomes-mediated co-delivery system. These methods are simple, reliable and accurate, which can be used in other similar experiments.
