Antibacterial mechanism of cryptotanshinone
- Author:
Chang-Qin LI
1
Author Information
1. Institute of Chinese Materia Medica
- Publication Type:Journal Article
- Keywords:
Antibacterial mechanism;
Cryptotanshinone;
Methcillin-resistant Staphylococcus aureus;
Staphylococcus aureus
- From:
Chinese Pharmaceutical Journal
2012;47(21):1706-1710
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the antimicrobial activity and antibacterial mechanisms of cryptotanshinone against SA, MRSA and ESBLS-SA. METHODS: MIC value was determined by disc diffusion method. IC50 value was determined by liquid culture. The antibacterial mechanisms of cryptotanshinone were investigated by determining the changes in electric conductivity, concentration of AKP, protein content, and the changes of protein electrophoretic bands in SDS-PAGE. RESULTS: The antibacterial rings, MIC and IC50 values showed that the antimicrobial activity against SA was better than that against MRSA and ESBLs-SA. Treated with cryptotanshinone, the electric conductivity, concentration of AKP, and protein content were increased, and protein bands in SDS-PAGE were changed obviously. CONCLUSION: Cryptotanshinone significantly inhibits SA, MRSA and ESBLs-SA and can damage the structures of cell wall and cell membrane, which results in the increase of permeability of cell membrane and release of cell components. Cryptotanshinone can influence the synthesis of bacteria protein, destroy the protein or reject the anabolism or expression of the protein, and finally leads to the loss of normal physiological function of bacterium.
