Screening and mutation of a marine Bacillus megaterium to resolute 3-chloro-1, 2-epoxypropane
- Author:
Dong-Sheng XUE
1
Author Information
1. Zhejiang University
- Publication Type:Journal Article
- Keywords:
3-chloro-1, 2-epoxypropane;
Marine microbe;
N+ ion;
Resolution;
Ultraviolet mutagenesis
- From:
Chinese Pharmaceutical Journal
2012;47(21):1694-1697
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To obtain and mutate a marine Bacillus megaterium to resolute pharmaceutical intermediate, 3-chloro-1,2-epoxypropane. METHODS: From marine sediment, a 3-chloro-1,2-epoxypropane-resolute strain was isolated and mutated by ultraviolet rays and N+ ion. RESULTS: The strain was identified as Bacillus megaterium on the basis of biophysical and biochemical test. The optimum salinity for growth was 2%, and the result of optimum salinity showed that the microbe was a typical marine microorganism. A positive mutant exhibited excellent resolution with ee value of 99% and yield of 42%. CONCLUSION: The marine bacillus megaterium shows a large potential for industrial production.
