Chemical constituents and quality standard of Girardinia diversifolia (Link) Friis
- Author:
Wen-Wei LIU
1
Author Information
1. Guiyang Medical University
- Publication Type:Journal Article
- Keywords:
β-sitosterol;
Girardinia diversifolia (Link) Friis;
HPLC;
Kaempferol;
Scopoletin;
TLC
- From:
Chinese Pharmaceutical Journal
2012;47(23):1943-1946
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the chemical constituents and quality standard of Girardinia diversifolia (Link) Friis. METHODS: The compounds were isolated by silica gel column chromatography and thin layer chromatography. Their structures were identified by physicochemical properties and various spectroscopic methods including NMR, MS, and UV, etc. Scopoletin in this preparation was identified by TLC. Its content was determined by HPLC. The separation of scopoletin was performed on a Shimadzu C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of acetonitrile (A)-0.2% phosphoric acid(B): 0-5 min, A-B (25: 75) →A-B(20: 80); 5-10 min, A-B(20: 80)→A-B(15: 85); 10-18 min, A-B(15: 85); 18-19 min, A-B(50: 50); 19-25 min, A-B (25: 75), with the flow rate of 1.0 mL · min-1, the UV detection wavelength was 298 nm, and column temperature was 30°C. RESULTS: Three compounds were isolated and identified as scopoletin (1), kaempferol (2), and β-sitosterol. They showed obvious characteristics in TLC identification. Good linearity was obtained over the range of 0.022-0.352jxg (r =0.999 9) for scopoletin. The average recovery (n=6) was 92.93% (RSD = 1.86%). CONCLUSION: Compounds 1-2 were isolated from the Girardinia diversifolia (Link) Friis for the first time. The qualitative and quantitative analysis methods are accurate and suitable for effective quality control of Girardinia diversifolia (Link) Friis.
