Rapid determination of ethanol extracts content of Pogostemon cablin (Blanco) Benth. by near-infrared spectroscopy
- Author:
Jia-Liang CAI
1
Author Information
1. School of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Ethanol extracts content;
Near-infrared spectroscopy;
Pogostemon cablin (Blanco) Benth.
- From:
Chinese Pharmaceutical Journal
2012;47(24):2026-2028
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To develop a method for the determination of ethanol extracts content of Pogostemon cablin (Blanco) Benth. using near-infrared spectroscopy. METHODS: The ethanol extracts contents of 139 samples were determined by the method of ethanol cold-soak extraction. Near-infrared spectrograms were obtained, then multiplicative signal correction, first derivative method and Savitzky-Golay filter method were used to process the spectrograms, at last the calibration model of ethanol extracts content was established by partial least squares regression analysis method. RESULTS: The correlation coefficients, root-mean-square error of calibration, root-mean-square error of prediction and root-mean-square error of cross-validation of the calibration model of ethanol extracts content were 0.98958, 0.590, 0.623 and 0.97518, respectively. CONCLUSION: The method is stable, accurate, and can be used to predict ethanol extracts content of Pogostemon cablin (Blanco) Benth. rapidly.
