Simultaneous determination of five components in Rehmanniae Radixby HPLC
- Author:
Jin YU
1
Author Information
1. Department of Chemistry
- Publication Type:Journal Article
- Keywords:
Acteoside;
Adenosine;
Catalpol;
Cistanoside A;
HPLC;
Leonuride;
Rehmanniae Radix
- From:
Chinese Pharmaceutical Journal
2013;48(1):73-76
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To develop an HPLC method for simultaneous determination of catalpol, leonuride, adenosine, cistanoside A and acteoside in Rehmanniae Radix. METHODS: The HPLC analysis was carried out on a Grace Allitma C18 column (4.6 mm × 250 mm, 5 μm), with a linear gradient elution of acetonitrile-water at the flow rate of 1.0 mL · min-1. The detective wave length was set at 203 nm, and the column temperature was set at 25°C. RESULTS: The calibration curves were linear within the range of 12.47-997.22 μg · mL-1 for catalpol, 2.85-227.78 μg · mL-1 for leonuride, 0.36-28.75 (μg · mL-1 for adenosine, 1.30-104.17 μg · mL-1 for cistanoside A, and 1.65-131.94 μg · mL-1 for acteoside, respectively. The average recoveries for the five marker compounds were 98.8%-100.0%, r =0.9997-1.0000. CONCLUSION: This method is simple, accurate and practical for the quality control of Rehmanniae Radix. There are some differences in the contents of the five marker compounds in the samples from different producing areas. Copyright 2013 by the Chinese Pharmaceutical Association.
