Study of Eu-metacycline complex fluorescent probe and its analytical application
- Author:
Jian-Yuan LIN
1
Author Information
- Publication Type:Journal Article
- Keywords: Cetyltriethyl ammnonium bromide; Europium (III); Fluorescence spectrome; Methacycline
- From: Chinese Pharmaceutical Journal 2013;48(2):143-147
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the luminescence effect of Eu3+-Metacycline (MTC)-cetyltrimethyl ammonium bromide (CTMAB) system and the luminescence mechanism of the system. METHODS: Based on the luminescence effect, a fluorescent method for the determination of MTC was proposed. Fluorescence characteristics of the complex of metacycline (MTC) with Eu3+ in a micellar system was studied, and the effect of different concentrations of MTC and MTC complexes on Escherichia coli and the Staphylococcus aureus in optimum experimental conditions was investigated. RESULTS: The experiments indicated that in pH 9.0-10.5 Na2B4O7-NaOH buffer solution, both MTC and cationic surfactant (CTMAB) could enhance greatly the fluorescence intensity of Eu3+. The fluorescence intensity was linearly related to the fleroxacin concentration in the range of 0.45-12 μg · mL-1. CONCLUSION: A new fluorescent method has been developed for the determination of MTC in pharmaceutical preparations. The detection limit was 0.015 3 μg · mL-1 and the RSD was 1.03%. The complexes of different concentrations of MTC and MTC have good antibacterial effect against Escherichia coli, and the antibacterial effect is the best when the MTC's concentration is 1 × 10-3 mol · L-1 against escherichia colon.
