Simultaneous determination of ligustilide, senkyunolide I and other six components in Shunaoxin dropping pills by UPLC-Q-TOF-MS
10.11669/cpj.2013.03.012
- Author:
Xiao-Qing YAN
1
Author Information
1. Tianjin Hospital
- Publication Type:Journal Article
- Keywords:
LC-MS;
Ligustilide;
Q-TOF-MS;
Senkyunolide I;
Shunaoxin dropping pill;
UPLC
- From:
Chinese Pharmaceutical Journal
2013;48(3):216-219
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an LC-MS method for simultaneous determination of six compounds (uridine, guanosine, ferulic acid, ligustilide, senkyunolide I and II in Shunaoxin dropping pills. METHODS: The analysis was performed on a Waters Acquity BEH C18 column (2.1 mm × 100 mm, 1.7 μm) with the mixture of acetonitrile-water-formic acid as the mobile phase, and the flow rate was 0.4 mL · min-1; the PDA detection wavelength was 190-400 nm; the column temperature was room temperature (30°C). RESULTS: The linear ranges for uridine, guanosine, ferulic acid, ligustilide, senkyunolide I and H were 10.31-206.24, 3.28-65.52, 6.36-127.24, 8.24-164.88, 10.18-200.52, and 3.14-62.72 μg · mL-1, respectively. The average recoveries were between 99%-101% (n=6). CONCLUSION: This method is simple, accurate and reliable to determine the contents of the six compounds in Shunaoxin dropping pills for the quality control.
