Identification of Velvet Antler by mitochondrial DNA fingerprint
10.11669/cpj.2013.03.003
- Author:
Yu-Juan GU
1
Author Information
1. School of Pharmacy
- Publication Type:Journal Article
- Keywords:
PCR;
R andom amplified polymorphic DNA (RAPD);
Specific primer;
Velvet Antler
- From:
Chinese Pharmaceutical Journal
2013;48(3):170-173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To compare specific primer method and r andom amplified polymorphic DNA (RAPD) method for identification of Velvet Antler, thus to choose a simpler method. METHODS: DNA samples were extracted from sika deer antler, wilddeer antler, reideer pilose antler and commercially available Velvet Antler, and then purified and amplified with specific primer amplification and RAPD amplification. RESULTS: The sika deer antler, wild deer antler, reideer pilose antler and some of the commercially available Velvet Antler all showed 313bp segment in the agarose gel with different brightness while the other commercial products did not show this segment. RAPD not only effectively showed positive and negative DNA amplification results, but also showed the difference in PCR products of sika deer antlerwild deer antler with different strip numbers and strip brightness. CONCLUSION: RAPD method is more accurate and rapid and it has wide application prospect.
