Determination of imatinib in rat plasma by HPLC and study of its pharmacokinetics
- Author:
Chen-Ming WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: HPLC; Imatinib; Pharmacokinetics; Plasma concentration
- From: Chinese Pharmaceutical Journal 2013;48(4):293-296
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To develop a high performance liquid chromatography method for the determination of imatinib in rat plasma and study its pharmacokinetics. METHODS: Plasma was deposited by perchloric acid. The analytical column was ZORBAX SB-C18 (4.6 mm × 150 mm, 5 μm). The mobile phase was acetonitrile-water-0.1% trifluoroacetic acid (19:61:20) and the flow rate was 1.0 mL · min-1. The LV detection wavelength was 282 nm. Six male SD rats were given a single dose of 50 mg · kg-1 imatinib by garage. Blood samples were collected from tail vein at different time points. The concentrations of imatinib in plasma were determined by the established HPLC method. The pharmacokinetic parameters were analyzed by DAS program. RESULTS: Excellent liner relationship was obtained in the range of 0.10-20.00 mg · L-1 (r=0.9998), and the lower limit of qauntification was 0.05 mg ·L-1. The recoveries were (98.86±2.77)%, (100.35±2.31)% and (100.14±1.66)% respectively at three concentrations (0.25, 5.00, 15.00 mg · L-1), the intra-day RSDs were 2.64%, 2.50% and 1.62% with REs of -1.60%, -1.00% and 0.10%, and the inter-day RSDs were 3.51%, 2.77% and 1.34% with REs of -0.80%, 0.72% and 0.21%, respectively. After oral administration in rats, the pharmacokinetic profile of imatinib was fitted with a two-compartment model, the half-lives of α phase and β phase were (2.40±0.84) h and (7.82±0.87) h, respectively. CONCLUSION: The method is accurate, simple, rapid and can be used to determine imatinib concentration in rat plasma and study its pharmacokinetics.
