Proliferation inhibition and apoptosis induction effect of dextran-magnetic layered double hydroxide-fluorouracil drug delivery system on human colon cancer cells SW480
- Author:
Yan-Hong LIU
1
Author Information
- Publication Type:Journal Article
- Keywords: Cell apoptosis; Cell proliferation; Colon cancer cell; DET-MLDH-FU drug delivery system; Magnetic layered double hydroxides
- From: Chinese Pharmaceutical Journal 2013;48(5):359-367
- CountryChina
- Language:Chinese
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Abstract:
OBJECTIVE: To explore the proliferation inhibition and apoptosis induction effect of dextran-magnetic layered double hydroxide-fluorouracil (DMF) drug delivery system on colon cancer cell SW480 cultivated in vitro. METHODS: MTT experiment was used to detect the cell proliferation inhibition rates of DET-MLDH-FU, MLDH-FU and MLDH supra-molecules at various concentrations. Light microscopy and Giemsa dyeing methods were applied for characterizing the shape change of the apoptotic cells. Flow cytometric analysis was employed to test the apoptosis rates of SW480 cells treated with different drugs for 24 h. DNA ladder method was used to analyze nuclei breakup of the apoptosis cells. RESULTS: The IC50 of MLDH, MLDH-FU and DMF three-level supra-molecules for SW480 cells were 44.83(25.74, 48.78), 15.16(13.78, 16.66) and 13.33 (11.03, 15.13) μg · mL-1, respectively. The proliferation of colon cancer cells SW480 was obviously restrained after being treated with 5-Fu and equivalent MLDH, MLDH-FU and DMF of different concentrations for 24, 48 and 72 h, respectively. The result revealed that the inhibition rates increased in the sequence of MLDH
