Pharmacokinetics of levofloxacin in rat brain and bloods
- Author:
Jia ZHOU
1
Author Information
- Publication Type:Journal Article
- Keywords: High performance liquid chromatography; Levofloxacin; Microdialysis; Pharmacodynamics; Pharmacokinetics
- From: Chinese Pharmaceutical Journal 2013;48(6):466-469
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To investigate the pharmacokinetics of levofloxacin in rats brain tissues and blood. METHODS: Brain tissues and blood were sampled by microdialysis simultaneously. The concentrations of levofloxacin in both blood and brain tissues were measured by high performance liquid chromatography. The concentration-time data was analyzed by WinNonlin software. RESULTS: The maximum concentrations of free levofloxacin in blood and brain tissues were reached at 10 min and 20 min, respectively, then both continuously decreased. The concentration of free levofloxacin in blood was higher than that in brain tissues in the first 4h, then returned to similar level. The area under the curve (AUC) of free levofloxacin was (21.17±7.83) μg · h · mL-1 in blood and (7.00±2.65) μg · h · mL-1 in brain tissues. CONCLUSION: Microdialysis with reversed phase high performance liquid chromatography established in this study can be used to determine the pharmacokinetics of levofloxacin in brain tissues and blood.
