A correlational study of three determination methods of mycophenolic acid blood concentrations in renal transplant patients
- Author:
Gang CUI
1
Author Information
- Publication Type:Journal Article
- Keywords: Correlation; HPLC; Mycophenolic acid; Plasma concentrations; UPLC-MS/MS
- From: Chinese Pharmaceutical Journal 2013;48(6):461-465
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To analyze the correlation between UPLC-MS/MS method and HPLC-UV method for determination of mycophenolic acid (MPA) blood concentrations in renal transplant patients and to establish a generally applicable conversion method of the determination results. METHODS: MPA concentrations in whole blood and plasma of transplant patients were determined by UPLC-MS/MS method. Plasma concentrations of MPA were determined by HPLC-UV. Variance analysis of the measure results was done by LEVENE and LSD methods, and the correlation between the methods was analyzed by Spearman method. RESULTS: Three conversion formula were obtained as follows; YUPLC_MS/MS plasma)=1.612 XUPLD_MS/M/Swhole blood+0.686, YUPLC_MS/MS plasma=1.414XUPLD_MS/M/S whole blood-0.206, and YHPLC-LV plasam=0.848 XUPLD_MS/M/S whole blood-0.692. CONCLUSION: There is good correlation between UPLC-MS/MS method and HPLC-UV method for the determination of MPA concentration in whole blood and plasma in renal transplant patients. The results can be transformed by equations.
