Research of determination methods of fluorouracil injection
- Author:
Chang-He WANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Double alcohol type; Double ketone type structure; Fluorouracil injection; HPLC; UV spectrophotometry absorption coefficient method
- From: Chinese Pharmaceutical Journal 2013;48(7):558-561
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To confirm the best content determination method of fluorouracil injection. METHODS: The contents of 95 batches of fluorouracil injection were determined by UV spectrophotometry absorption coefficient method and high performance liquid chromatography (HPLC), respectively. Through investigation of method recoveries, the reasons for difference in the content determination results were further analyzed. RESULTS: The HPLC determination results were slightly higher than those of the UV method (P<0.05), The intervonversion of double alcohol type structure and double ketone type structure of fluorouracil might lead to the lower recovery and determination results of UV method. CONCLUSION: The HPLC method is more accurate and reliable than UV method qualitatively and quantitatively.
