Tissue distribution study of capsosomes in vivo and evaluation of doxorubicin-loaded capsosomes in vitro
- Author:
Hui SHI
1
Author Information
- Publication Type:Journal Article
- Keywords: Capsosome; Cytotoxicity; Doxorubicin; Tissue distribution
- From: Chinese Pharmaceutical Journal 2013;48(7):536-540
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the tissue distribution of capsosomes in vivo and the efficacy of DOX-loaded capsosomes in vitro. METHODS: Cy5-Capsosomes (capsosomes encapsulating Cy5-BSA molecules) were prepared by co-precipitation method and layer-by-layer assembly technique. After intravenously injected into nude mice via the tail vein, the tissue distribution of Cy5-capsosomes at defined time points were studied under Maestro in-vivo imaging system. The cytotoxicity of DOX-loaded capsosomes for B16F10 cells was identified by MTT, the apoptosis induced by DOX-loaded capsosomes was observed by means of fluorescent microscopy. RESULTS: Cy5-Capsosomes achieved in the lung less than 1h and accumulated there for more than 12h. There was little Cy5-Capsosomes distributed in other organs. Capsosomes had low toxicity, while the DOX-loaded capsosomes had strong toxicity for B16F10 cells and induced apoptosis obviously. CONCLUSION: The capsosome had favourable passive pulmonary targetability, and it can be used as a novel drug carrier in potential.
