Structure characterization of extracellular polysaccharide of Phellinus pini
- Author:
Lei YUAN
1
Author Information
- Publication Type:Journal Article
- Keywords: Phelinus pini.; Polysaccharide; Structure analysis
- From: Chinese Pharmaceutical Journal 2013;48(8):597-600
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To characterize the structure of extracellular polysaccharide of Phellinus pini. METHODS: The purity and molecular weight of polysaccharide were determined by HPLC, GC, IR, complete acid hydrolysis, periodate oxidation, Smith degradation, methylation analysis and 13C-NMR were applied to determine the structural features. RESULTS: Extracellular polysaccharide of Phellinus pirn (PPE) had uniform component, and the content of polysaccharide was 92.84%. The molecular weight was 3.9 × 104. PPE was composed of Man, Glc and Gal with the molar ratio of 38.4:1.76:1. The backbone of PPE was composed of α-(1→2)-Man. The branches were located at O-6 of α-(1→2)-Man. The branched chain was consisted of α-(1→3)-Man and α-(1→6)-Man. The terminal sugar was Man. CONCLUSION: This is the first in-depth study on the characterization of extracelular polysaccharide of Phelinus pini.
