Simultaneous determination of six flavonoid active components in Radix Astragali by UPLC
- Author:
Juan FU
1
Author Information
- Publication Type:Journal Article
- Keywords: Astragalus membranaceus; Campanulin; Content determination; Ononin; Quercetin; UPLC
- From: Chinese Pharmaceutical Journal 2013;48(11):916-919
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish an UPLC method for simultaneous determination of six flavonoid active components (campanulin, ononin, quercetin, calycosin, kaempferol, and formononetin) of the roots of Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao collected from the main producing areas (Gansu Province, Shaanxi Province, and Inner Mongolia). METHODS: Separation was performed at 30°C on an ACQUITY UPLC BEH C18 column (2.1 mm ×100 mm, 1.7 μm) with a gradient elution system of water containing 0.1% formic acid (mobile phase A) and acetonitrile - isopropanol (7:3) containing 0.1% formic acid (mobile phase B). The flow rate was 0.25 mL·min-1. The detection wavelength was 254 nm, and the sample injection volume was 2 μL. RESULTS: The six flavonoid active components showed good linearity in the selected concentration ranges (r≥0.999), with average recoveries of 99.60%-101.2% (n=6) and RSDs of 1.2%-2.0%. CONCLUSION: This study for the first time establishes an UPLC method for simultaneous determination of six flavonoid active components (campanulin, ononin, quercetin, calycosin, kaempferol, and formononetin) of the roots of Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao. This method is specific, reproducible, controllable, and can be used for the quality control of Radix Astragali.
