Simultaneous determination of five nucleosides in Antrodia camphorata by HPCE
- Author:
Feng-Su ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Antrodia camphorata; HPCE; Nucleobase; Nucleoside; Simultaneous determination
- From: Chinese Pharmaceutical Journal 2013;48(12):1018-1021
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish a high performance capillary electrophoresis (HPCE) method for simultaneous determination of five nucleosides and nucleobases, including adenine, adenosine, uridine, guanosine and inosine in antrodia camphorate of different batches. METHODS: Based on the mode of HPCE, uncoated fused silica capillary (75 μm×64.5 cm, 56 cm of effective length) was used with separation voltage of 22 kV. 60 mmol·L-1 sodium borate was selected for the running buffer solution (pH 9.3). The detection wavelength was set at 260 nm. The sample was injected at 50 mbar×6 s and column temperature was maintained at 28°C. RESULTS: The calibration curves of the five nucleosides showed good linearity (r>0.9995). The average recoveries of the method were between 98.83%-101.08%. The contents of the five nucleosides in Antrodia camphorate samples of different batches were different. CONCLUSION: The established method is reliable, accurate and can be used for the quality control of Antrodia camphorata.
