Effect of combination valdecoxib and pirarubicin on promoting apoptosis in human lung cancer cell
- Author:
Qing-Hua XU
1
Author Information
- Publication Type:Journal Article
- Keywords: Apoptosis; Human lung cancer A549 cell line; Pirarubicin; Valdecoxib
- From: Chinese Pharmaceutical Journal 2013;48(12):972-975
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the effect of valdecoxib and pirarubicina combination on cell cycle and apoptosis of human lung cancer A549 cell line in vitro. METHODS: MTT assay was used to analyze the effect of valdecoxib and pirarubicina on the growth of human lung cancer A549 cell line. The median-effect principle was applied to determine the combination effect of valdecoxib and pirarubicina. Flow cytometry (FCM) was used to observe the cell cycle and apoptosis. The expressions of Bcl-2, Bax, and Caspase-3 were detected by western blotting. RESULTS: The inhibition ratio of A549 cell in valdecoxib and pirarubicina combination group was increased compared with their respective application, and CI<1. In valdecoxib and pirarubicina combination group, the apoptosis rate and the expression of Bax and Caspase-3 was increased, and the expression of Bcl-2 was decresed. CONCLUSION: Valdecoxib and pirarubicina combination has synergistic effect, which is partly due to the increase of the apoptosis rate.
