Anti-Hepatoma activity of wogonin on HepG2 cell in vitro
- Author:
Yu-Jian ZHONG
1
Author Information
- Publication Type:Journal Article
- Keywords: Anti-tumor; Apoptosis; Hepatoma; HepG2; Wogonin
- From: Chinese Pharmaceutical Journal 2013;48(12):968-971
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the growth inhibitory and apoptosis-inducing effect of wogonin on hepatocarcinoma cell line HepG2. METHODS: HepG2 cell line was treated with various concentrations of wogonin. Proliferation was determined by MTT test and morphology observation. Flow cytometry was applied to detect the wogonin-induced apoptosis and to analyse the cell cycle. Western blot was used to detect HDAC1 and HDAC2 expression. RESULTS: Wogonin had a growth inhibitory and apoptosis-inducing effect on HepG2 cell. The cell showed G2/M arrest by cell cycle analysis. A characteristic apoptosis change including nuclear disintergration and chromatin agglomerate was displayed. Western blot results show increase of cleave PARP, but not change in HDAC1, HDAC2 expression. CONCLUSION: Wogonin has a growth inhibitory and apoptosis-inducing effect on hepatocarcinoma cell line HepG2.
