Determination of four saccharides in Qingkailing injection by HPLC-ELSD
- Author:
Hong-Xia ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Cluster analysis; Fructose; Glucose; HPLC-ELSD; Qingkailing injection; Sucrose; Xylose
- From: Chinese Pharmaceutical Journal 2013;48(14):1183-1186
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish an HPLC-ELSD method to determine the contents of four saccharides in Qingkailing injection and carry out cluster analysis of the products from different manufactures. METHODS: Chromatographic separation was performed on a Prevail Carbohydrate ES column with mobile phase of acetonitrile (B) - water (A) at a flow rate of 1.0 mL·min-1 with gradient elution; the column temperature was 30°C; the ELSD drift tube temperature was 90.7°C and the gas flow was 2.4 L·min-1. Cluster analysis was performed by SPSS16.0. RESULTS: Good resolution and linearities were obtained. The average recoveries of xylose, fructose, glucose and sucrose were 98.52% (RSD=1.70%), 99.15% (RSD=2.55%), 99.82% (RSD=2.04%) and 99.26% (RSD=1.67%), respectively. The test samples were clustered into three classes. CONCLUSION: The established method is simple, reliable and accurate. It can be used to determine the saccharide components in Qingkailing injection, and provide theoretical basis for its quality control.
