Research progress in receptor binding assay by mass spectrometry
- Author:
Ai-Ying KONG
1
Author Information
- Publication Type:Journal Article
- Keywords: Dissociation constant; Ligand receptor binding assay; Mass spectrometry; MS binding assay; Specific binding
- From: Chinese Pharmaceutical Journal 2013;48(15):1244-1248
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To explain that receptor-ligand binding assay is an important drug screening method, which can quickly and inexpensively study the interactions between the targeted receptor and the potential ligands in vitro and provide information about the relative binding affinity of ligand-receptor. METHODS: The correlative literature and abroad were collected and analyzed. RESULTS AND CONCLUSION: The concept of MS binding assay based on mass spectrometric quantification of nonlabeled markers represents a promising alternative to conventional radioligands binding without inherent drawbacks. The technique will play an important role in the design and screening of receptor-targeting pharmaceuticals.
