Determination of bivalirudin in human blood by HPLC-MS/MS
- Author:
Ka-Xu YANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Bivalirudin; HPLC-MS/MS; SPE
- From: Chinese Pharmaceutical Journal 2013;48(16):1400-1403
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish an HPLC-MS/MS method for the determination of blood-bivalirudin concentration. METHODS: Following a SPE method, the analytes were separated on a C18 column interfaced with a triple-quadrupole tandem mass spectrometer using positive electrospray ionization. RESULTS: The calibration linear rang of blood-bivalirudin concentration was 25-20000 μg · L-1 (r = 0.9969). The between-day and within-day RSD% were less than 6.63%. The recoveries were more than 80%. CONCLUSION: The rapid, sensitive and reliable HPLC-MS/MS method can be applied to monitor blood- bivalirudin profile.
