Determination of D(-) sulbenicillin sodium in sulbenicillin sodium for injection by HPLC-MS
- Author:
Fang-Wen SHUAI
1
Author Information
- Publication Type:Journal Article
- Keywords: D(-) sulbenicillin; HPLC-MS; Quantitative analysis; Sulbenicillin sodium for injection
- From: Chinese Pharmaceutical Journal 2013;48(22):1951-1954
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish a method for determination of D(-) sulbenicillin sodium in sulbenicillin sodium for injection by HPLC/MS. METHODS: The analysis was performed on a SHIMADZU VP-ODS column with mobile phase consisting of 0.01 mol·L-1 ammonium acetate solution-methanol (90:10) at a flow rate of 0.6 mL·min-1. The detection was performed by Multiple Reaction Monitoring (MRM) scanning mode with ESI negative ion mode. RESULTS: D(-) and L(+) sulbenicillin could be effectively separated. Good liner relationship was achieved when the concentration of D(-) sulbenicillin was in the range of 1.03-51.90 μg· mL-1 (r = 0.9997). The average recovery rate was 99.98% at three levels (RSD = 0.30%, n = 9). CONCLUSION: The MRM method is accurate, reliable, and can be used for the determination of D(-) sulbenicillin sodium in sulbenicillin sodium for injection.
