Effect of Rhaponticum uniflorum on hepatic oxidative stress and DNA damage induced by carbon tetrachloride
- Author:
Hao SONG
1
Author Information
- Publication Type:Journal Article
- Keywords: Carbon tetrachloride (CCl4); DNA damage; Liver injury; Oxidative stress; Rhaponticum uniflorum
- From: Chinese Pharmaceutical Journal 2013;48(22):1915-1918
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To investigate the effect of Rhaponticum uniflorum water extract (RUWE) on the oxidative stress and DNA damage of liver with an acute liver injury induced by carbon tetrachloride (CCl4) in mice. METHODS: Mice were randomly assigned to the normal control, model, bifendate (BFD), as well as high and low dose groups of RUWE. Animals were treated once daily for 7 d. At the end of the experiment, CCl4 was given intraperitoneally to the mice of groups, then the alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipid hydroperoxide (LOOH), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), Na+-K+-ATPase and Ca2+-Mg2+-ATPase were detected with the colorimetric method, and DNA damage was analyzed using the electrophoretic method. RESULTS: The administration with RUWE reduced the serum activities of ALT and AST, decreased the LOOH and MDA contents of liver, increased the CAT, GSH-Px, total SOD, Mn-SOD activities and GSH level of liver, increased the Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities of liver mitochondria, and reduced the DNA damage of hepatocyte in mice with acute liver injury. CONCLUSION: RUWE has protective effect on acute liver injury induced by CCl4 in mice, probably via reduction of the oxidative stress and DNA damage of liver tissue.
