Rapid determination of six constituents in Tongxuanlifei concentrated pills by HPLC
- Author:
Jun ZHOU
1
Author Information
- Publication Type:Journal Article
- Keywords: Baicalin; Glycyrrhizic acid; Hesperidin; HPLC; Multicomponent assay; Naringin; Neohesperidin; Quality control; Rapid determination; Rosmarinic acid; Standard improvement; TCM preparation; Tongxuanlifei concentrated pills
- From: Chinese Pharmaceutical Journal 2013;48(21):1856-1859
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To develop an HPLC method for the rapid determination of naringin, hesperidin, neohesperidin, rosmarinic acid, baicalin and glycyrrhizic acid in Tongxuanlifei concentrated pills(traditional Chinese medicines). METHODS: The analysis was carried out on an Agilent Porlshell 120 SB-C18 column (4.6 mm × 250 mm, 2.7 μm). The mobile phase was composed of 70% acetonitrile (A) and 0.1% phosphoric acid aqueous solution (B) with gradient elution(0 - 20 min, 25% A→30% A; 20 - 25 min, 30% A→95% A; 30 - 31 min, 95% A→25% A; 31 - 40 min, 25% A) and the flow rate was 1.0 mL·min-1. The detection wavelengths were set at 283 nm for naringin, hesperidin, neohesperidin (0 - 10.8 min), 330 nm for rosmarinic acid (10.9 - 12 min), 276 nm for baicalin (12.1 - 20 min) and 250 nm for glycyrrhizic acid (20.1 - 45 min). The column temperature was 30°C. RESULTS: The linear ranges of naringin, hesperidin, neohesperidin, rosmarinic acid, baicalin and glycyrrhizic acid were 0.00569 - 0.8532 μg, 0.00325 - 0.4879 μg, 0.00365 - 0.5472 μg, 0.00051 - 0.0762 μg, 0.00796 - 1.1939 μg, and 0.00558-0.8377 μg (r≥ 0.9990), respectively. The average recoveries were 98.02%, 96.53%, 96.44%, 96.74%, 97.56% and 98.57% (RSD < 2.0%, n = 6), respectively. CONCLUSION: This method is simple and can be used to determine the above-mentioned six components with satisfactory accuracy and repeatability.
