Effect of cinobufacini injection on proliferation and tube-like structure formation of human lymphatic endothelial cells co-cultured with human hepatoma HepG-2 cells
- Author:
Xiu-Ying CHEN
1
Author Information
- Publication Type:Journal Article
- Keywords: Cinobufacini injection; HepG-2 cell; HLEC; Migration; Proliferation; Tube-like structure formation
- From: Chinese Pharmaceutical Journal 2013;48(21):1837-1840
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study the effect of cinobufacini injection on proliferation, migration and tube-like structure formation of human hepatoma HepG-2 cells co-cultured with human lymphatic endothelial cells. METHODS: Co-culture system of human hepatoma HepG-2 cells and human lymphatic endothelial cells is established by means of transwell chamber; cell growth curve is used to observe the effect of cinobufacini injection on the proliferation of HLEC co-cultured with HepG-2 cells; migration assay is used to observe the effect of cinobufacini injection on the migration of HLEC co-cultured with HepG-2; matrigel assay is used to observe the effect of cinobufacini injection on the tube-like structure formation of HLEC co-cultured with HepG-2 cells. RESULTS: Cinobufacini injection significantly inhibits proliferation(P < 0.05), migration (P < 0.05) and tube-like structure formation (P < 0.05) of HLEC co-cultured with HepG-2 cells in dose dependent ways. CONCLUSION: Cinobufacini injection inhibits proliferation, migration and tube-like structure formation of HLEC co-cultured with HepG-2 cells, which might contribute the inhibiting mechanisms of cinobufacini injection on tumor metastasis.
