Optimization of the prescription of Calcein-loaded novel temperature-sensitive liposomes
10.11669/cpj.2013.23.012
- Author:
Chao-Qin HE
1
Author Information
1. College of Pharmacy
- Publication Type:Journal Article
- Keywords:
Calcein;
Lysophospholipid;
Novel temperature-sensitive liposome;
Release
- From:
Chinese Pharmaceutical Journal
2013;48(23):2022-2025
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To optimize the prescription of the novel temperature-sensitive liposomes(NTSL). METHODS: Calcein-loaded temperature-sensitive liposomes with different phospholipid composition (DPPC-MSPC-DSPE-PEG200=86:10:4 and N (20% HSPC + 80% DPPC) -MSPC-DSPE-PEG200=92:4:4) were prepared by the thin film -hydration method. Based on the fluorescence self-quenching off characteristics of calcein, the release behavior of the above liposomes was compared when exposed to 37°C (normal temperature) as well as their respective phase transition temperature (Tm). RESULTS: Compared with the traditional temperature-sensitive liposomes (TTSL), the NTSL showed more favorable temperature-sensitive release performance and more stable encapsulation ability to calcein, even with the amount of lysophospholipids was reduced by 60 percent. CONCLUSION: The application of NTSL helps to enhance the effect of temperature-sensitive release.
