An in vitro analytical method based on MTT assay for the determination of dissolution rate of Fufangbiejiaruangan tablet
- Author:
Ling DAI
1
Author Information
- Publication Type:Journal Article
- Keywords: Dissolution rate; Fufangbiejiaruangan tablet; LX-2; MTT assay
- From: Chinese Pharmaceutical Journal 2013;48(23):2018-2021
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To explore the new pattern of Chinese medicine solid preparations (CMSP) in vitro dissolution, a method testing the cell inhibition rate in combination with HPLC was used. METHODS: MTT assay was used to obtain the characteristic cell inhibition rate by Fufangbiejiaruangan tablet dissolving solutions at the pH 7.4 (phosphate buffer) dissolution medium at different times. From these results, the cumulative dissolution of Fufangbiejiaruangan tablets based on cell inhibition rate was obtained. The dissolution rate of paeoniflorin was determined by HPLC method. Then f2 similar factor method was used to evaluate the relevance of these two methods. RESULTS: f2 values was less than 50, indicating that there is a poor correlation between the two methods of measuring the dissolution rate. It is unfeasible to determine CMSP in vitro dissolution just by using paeoniflorin cumulative dissolution. CONCLUSION: Biological testing is expected to become CMSP in vitro dissolution of one of the evaluation tools.
