Determination of patchouli alcohol content in Huodan pills by near-infrared spectroscopy
- Author:
Sheng-Guo JI
1
Author Information
- Publication Type:Journal Article
- Keywords: GC; Huodan pill; NIRS; Patchouli alcohol; Quantitative analysis
- From: Chinese Pharmaceutical Journal 2013;48(24):2154-2157
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To determine the content of patchouli alcohol in Huodan pills (traditional Chinese medicine) by near-infrared (NIR) spectroscopy. METHODS: The content of patchouli alcohol in 147 batches of Huodan pills samples were determined by GC. NIR spectra of all samples were collected, then standard normal variate, first derivative and Norris derivative filter methods were used as spectral preprocessing tools, and at last, the calibration model of patchouli alcohol content was established by partial least squares regression. RESULTS: The correlation coefficients, the root-mean-square error of calibration, and the root-mean-square error of cross-validation of the calibration model for patchouli alcohol were 0.95509, 0.0315, and 0.04510, respectively; the root-mean-square error of prediction and the average recovery rate of validation samples were 0.0343 and 100.76%, respectively. CONCLUSION: The method is stable, accurate, and can be used to determine the content of patchouli alcohol in Huodan pills rapidly.
