Phenolic acids excretion after intravenous administration of Dengzhanxixin injection in rats
10.11669/cpj.2013.24.017
- Author:
Guo-Liang DAI
1
Author Information
1. College of Pharmacy
- Publication Type:Journal Article
- Keywords:
1, 3-dicaffeoylquinic acid;
Caffeic acid;
Chlorogenic acid;
Dengzhanxixin injection;
HPLC;
Phenolic acid;
Urine
- From:
Chinese Pharmaceutical Journal
2013;48(24):2141-2145
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To develop a HPLC method for the determination of the concentration of caffeic acid, chlorogenic acid and 1, 3-dicaffeoylquinic acid in rat urine and to observe the excretion of three compounds in rats. METHODS: Rat urine samples, collected at different intervals after vein injection of Dengzhanxixin injection traditional Chinese medicines, added with protocatechuic aldehyde as internal standard, handled by ethyl acetate, then were measured by HPLC. RESULTS: The recoveries of caffeic acid, chlorogenic acid and 1, 3-dicaffeoylquinic acid were about 95%-98%, 90%-95%, 95%-97%, respectively. The cumulative excretions of caffeic acid, chlorogenic acid and 1, 3-dicaffeoylquinic acid in urine within 60 h were (70.97 ± 7.31)%, (87.62 ± 12.23)% and (9.87 ± 2.63)%. Relative standard deviation(RSD) of intra- and inter-day assay was less than 10%. CONCLUSION: The established method is simple, accurate and specific. It can be successfully used for the analysis of three compounds in rat urine.
