Effect of Tibetan medicine Oxytropis falcata on cell apoptosis and expression of Bcl-2 and Bax protein in SMMC-7721 cells
- Author:
Guang-Ming YANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Anti-tumor; Apoptosis; Oxytropis falcata; Protein expression; SMMC-7721
- From: Chinese Pharmaceutical Journal 2013;48(24):2113-2116
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To probe the influence of Oxytropis falcata on apoptosis and expression of Bcl-2 and Bax proteins in SMMC-7721 cells. METHODS: Annexin V-FITC/ PI assay was applied to detect apoptosis in SMMC-7721 cells after being treated with TFOF (total flavonoids of O. falcata). Changes of Bcl-2 and Bax expression were investigated by Western blot. RESULTS: Cell apoptosis was induced significantly in SMMC-7721 cells when subjected to TFOF. Western blot showed that Bcl-2 expression was downregulated. Although Bax expression was also downregulated slightly comparing with that of control, the ratio of Bcl-2/Bax decreased significantly after TFOF treatment. CONCLUSION: The results suggest that TFOF of O. falcata may be a pro-apoptotic agent for hepatic cancer cells via its modulation of Bcl-2/Bax balance.
