Purification and biological activity of recombinant human β-NGF expressed in E. coli

Author: Jia-Nan LI ¹
Author Information

1. Department of Biotechnology, Life Science College, Jianghan University
Publication Type:Journal Article
Keywords: Codon optimization; Escherichia coli expression system; Leading peptide; Nickel affinity chromatography column
From: Chinese Pharmaceutical Journal 2014;49(20):1785-1790
CountryChina
Language:Chinese
Abstract: OBJECTIVE: To express the recombinant human nerve growth factor by using codon mutation of Escherichia coli(rhβ-NGF), separate and purify the expression products, and determine the biological activity.