Sonication Induces Apoptosis in HL-60 Cells.
- Author:
Moonyoung CHOI
1
;
Incheol SEO
;
Seong Il SUH
;
Min Ho SUH
;
Won Ki BAEK
Author Information
1. Department of Microbiology, Keimyung University School of Medicine, Daegu, Korea. wonki@dsmc.or.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Leukemia;
Lysosome;
Sonication;
Ultrasonic therapy
- MeSH:
Apoptosis*;
Blotting, Western;
Cell Death;
Cell Line;
Flow Cytometry;
HL-60 Cells*;
Humans;
Leukemia;
Lysosomes;
Rupture;
Sonication*;
Ultrasonic Therapy;
Ultrasonography
- From:Keimyung Medical Journal
2014;33(2):109-119
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To investigate a specific mechanism of apoptosis induced by sonication, we applied 20 kHz ultrasound to leukemia cell line HL-60 with different intensities (0-60 W/cm2) and time durations (0-100 sec). In accordance with previous reports, ultrasound treatment in HL-60 cells induced immediate cell death and delayed cell death which are associated with cell lysis and apoptosis, respectively. Delayed cell death of HL-60 was also detected 5 hours after sonication in our experiment. Detection of caspase activation by Western blot and sub-G1 accumulation by flow cytometry confirmed that apoptosis plays a role in delayed cell death induced by sonication in HL-60 cells. In addition, we found that decrease in lysosomes of HL-60 cells after sonication suggesting lysosomal rupture is involved in the mechanism of cell death induced by sonication.
