Determination of chamaechromone in rat urine and feces by HPLC-MS and the excretion study
- Author:
Yan LOU
1
Author Information
- Publication Type:Journal Article
- Keywords: Chamaechromone; Excretion; Fece; HPLC-MS; Rat; Urine
- From: Chinese Pharmaceutical Journal 2014;49(1):68-75
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To develop a new method for the determination of chamaechromone in urine and feces of rats and investigate its excretion characteristics. METHODS: A liquid chromatography tandem mass spectrometry (LC-MS) method was developed. Chromatographic separation was performed on Xbridge™G18 column (2.1 mm×50 mm, 3.5 μm) with linear gradient elution u-sing water and methanol, both of which were acidified with 0.1% aqueous formic acid. The flow rate was 0.4 mL·min-1. Detection was performed on a triple-quadrupole tandem mass spectrometer using positive ion mode electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode. The monitored MS/MS ion transitions were m/z 543.3→198.9 and 481.9→258.3 for chamaechromone and rosuvastatin, respectively. RESULTS: Good linearity was observed over the concentration range of 8-6 400 ng·mL-1 in 0.1 mL of both rat urine and feces. The limit of detection (LOD) was 4 ng·mL: The intra-assay and inter-assay variabilities were less than 14% in both urine and feces. Cumulative urinary and fecal excretion ratios within 48 h at a dose of 100 mg·kg-1 after oral administration of chamaechromone accounted for (1.84±0.290)% and (41.5±6.917)% of the dose respectively. CONCLUSION: This method shows excellent sensitivity, precision, and accuracy, and is successfully applied to evaluate the excretion of chamaechromone in urine and feces of rats. The results indicate that feces are the major excretion route of chamaechromone after oral administration. And the most efficient excretion happens in 12-24 h after administration.
