Effects of Bauhinia championii flavones on hypoxia-reoxygenation injury in myocardial cells
- Author:
Wei-Xin LIN
1
Author Information
- Publication Type:Journal Article
- Keywords: Bauhinia championii flavones; Cradiomyocyte; Hypoxia/reoxygenation injury
- From: Chinese Pharmaceutical Journal 2014;49(1):36-39
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To observe the effect and mechanisms of Bauhinia championii flavones(BCF) on hypoxia/reoxygenation (H/R) myocardial cells of neoanatal rats. METHODS: The cardiomyocytes hypoxia/reoxygenation injury model was developed, and Bauhinia championii flavones was pretreated (final concentration as 50, 100, 200 mg·L-1 respectively). The morphological changes of cardiomyocytes were observed by invert microscope. ELISA was used to evaluate the activities of tumor necrosis factor alpha, the protein expression of Bcl-2, Bax and NF-κβ was observed by immunohisto-chemistry, and Annex v-FITC/PI staining was used to detect apoptosis rate. RESULTS: Compared with model group, Bauhinia championii flavones pretreatment eased cardiomyocytes injury, decreased the activities of TNF-α and iNO S, maintained the eNOS level, up-regulated the expression of Bcl-2, down-regulated Bax and NF-κβ, and inhibited cadiocyte apoptosis (P<0.01 or P<0.05). CONCLUSION: BCF has protective effects on hypoxia/reoxygenation injury, which are associated with adjusting iNOS and NF-κβ signal channel, up-regulating Bcl-2, down-regulating Bax and NF-κβ, and inhibiting cadiocyte apoptosis.
