Simultaneous determination of nine components in Zhibaidihuang concentrated pills by HPLC-MS/MS
- Author:
Ying-Guang SUN
1
Author Information
- Publication Type:Journal Article
- Keywords: Determination; HPLC-MS/MS; Mangiferin; Neomangiferiu; Zhibaidihuang concentrated pill
- From: Chinese Pharmaceutical Journal 2014;49(2):152-155
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To develop a method for the determination of neomangiferin, mangiferin, timosaponin BII, timosaponin A III, berberine, morroniside, loganin, paeoniflorin, and paeonol in Zhibaidihuang concentrated pills (traditional Chinese medicine) by HPLC-MS. METHODS: The samples were separated on a Diamonsil C18 column (4.6 mm × 150 mm, 5 μm) by gradient elution using acetonitrile and 0.1% aqueous formic acid as the mobile phase. The column temperature was 30°C. The elution program was optimized as follows; 0-2.5 min, linearly changed from A-B (5:95, V/V) to A-B (55:45, V/V); 2.5-5 min, linearly changed from A-B (55:45, V/V) to A-B (95:5, V/V); and 5-15 min, isocratic elution A-B (95:5, V/V); then quickly returned to initial A-B (5:95, V/V). This was followed by an equilibration period of 6 min prior to injection of next sample. The flow rate of mobile phase was set at 0.8 mL · min-1, and the injection volume was 10 μL. Multiple-reaction monitoring (MRM) scanning was employed for quantification with switching electrospray ion source polarity between positive and negative modes in a single run. RESULTS: There was significant correlation between the peak area and concentration of each compound within the test range (r > 0.9993). The average recoveries (n=3) of the nine compounds were 98.33%, 99.73%, 100.1%, 101.2%, 101.2%, 100.2%, 99.90%, 100.6% and 100.5%, respectively. The RSDs were 1.99%, 1.57%, 1.21%, 2.73%, 1.76%, 1.59%, 1.11%, 1.70% and 1.05%, respectively. CONCLUSION: The method is simple, accurate and highly reproducible, and can be used for the determination of nine compounds in Zhibaidihuang concentrated pills.
