Optimization of immobilized effect of the O-carboxymethyl chitosan multi-hemostatic sponge on thrombin by the central composite design-response surface methodology
- Author:
Di WU
1
Author Information
- Publication Type:Journal Article
- Keywords: Activity yield; Central composite design-response surface methodology; Immobilization; O-carboxymethyl chitosan; Thrombin
- From: Chinese Pharmaceutical Journal 2014;49(2):138-142
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To investigate the effects of O-carboxymethyl chitosan (O-CMC) multi-hemostatic sponge on the immobilization of thrombin. METHODS: The formulation of O-CMC multi-hemostatic sponge was optimized by using central composite design-response surface methodology (CCD-RSM), in which the O-CMC, glutaraldehyde and thrombin were used as enzyme immobilization carrier, crosslink agent and coagulant, respectively. The crosslinked conditions of thrombin were studied by determining the enzymatic activity yield. RESULTS: The optimized concentrations of O-CMC, thrombin and glutaraldehyde were 2.49%, 49.87 U · mL-1, 0.49%, respectively. CONCLUSION: O-CMC multi-hemostatic sponge has the positive immobilization effect on thrombin.
