Identification and quality evaluation of radix et Rhizoma Rhei by HPLC fingerprint and chemometrics analysis
- Author:
Yu PANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Anthraquinone; Chemometrics; Fingerprint; High performance liquid chromatography; Quality evaluation; Radix et Rhizoma Rhei
- From: Chinese Pharmaceutical Journal 2014;49(4):287-293
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To develop the HPLC fingerprint of Radix et Rhizoma Rhei and chemometrics analysis method. METHODS: The samples were extracted by ultrasonic with 70% ethanol, purified by 0.45 μm filter membrane, and eluted by 0.1% phosphoric acid and acetonitrile in gradient mode; reference substances and LC-MS were used to identify the chromatography peaks; the HPLC fingerprints of Radix et Rhizoma Rhei and its decoction pieces at 254 and 430 nm were compared and analyzed with CHROMAP software. RESULTS: Authentic and unauthentic Radix et Rhizoma Rhei all have their own characteristic peaks at 254 nm, but the characteristic peaks decreased at 430 nm and the proportion of common peaks areas were obviously different. Twenty-five chromatographic peaks at 254 nm and 19 peaks at 430 nm were selected to distinguish the authentic and unauthentic Radix et Rhizoma Rhei by similarity analysis as well as to evaluate the quality of crude drugs and decoction pieces. CONCLUSION: This method can be used for the identification and quality evaluation of different species of Radix et Rhizoma Rhei crude drugs and decoction pieces.
