Study on identification of cordyceps-a Chinese traditional medicine
- Author:
Hong XU
1
Author Information
- Publication Type:Journal Article
- Keywords: Cordyceps; DNA molecular marker; Identification; Pharmacognosy
- From: Chinese Pharmaceutical Journal 2014;49(4):283-286
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To study on the identification of Cordyceps and establish its traditional pharmacognostic identification and modern identification method based on DNA molecular marker technology. METHODS: The methods of macroscopic, microscopic examination and RAPD-SCAR were employed here to authenticate Cordyceps. RESULTS: Based on the macroscopic identification, the features of transverse section and powder of Cordyceps were described in detail. The digital photographs were presented here in repealing the main macroscopic and microscopic characteristic of Cordyceps. The specific RAPD fragment of Cordyceps was conversed into SCAR marker, and Cordyceps could be identified by the optimized PCR conditions. CONCLUSION: The authenticated method of microscopic and macroscopic identification is intuitive. However, the identification method based on DNA molecular marker is sample, which provides the new scientific evidences for the identification of authenticity of Cordyceps.
