In virro antifungal activity of 2-nonylamino-6-methoxytetralin hydrochloride
- Author:
Rong-Mei LIANG
1
Author Information
- Publication Type:Journal Article
- Keywords: 2-nonylamino-6-methoxytetralin hydrochloride; Antifungal activity; In vitro
- From: Chinese Pharmaceutical Journal 2014;49(6):449-454
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To investigate the in vitro antifungal activity of 2-nonylamino-6-methoxytetralin hydrochloride (10b). METHODS: The antifungal activities of 10b against 19 Candida albicans (C albicans) strains and 15 non-C albicans strains were assayed by microbroth dilution in 96-well plates according to the methods recommended by Clinical and Laboratory Standards Institute (CLSI). The sensitivity of C. albicans strain Y0109 to 10b and fluconazole (FLC) was compared by agar disk diffusion test, plotting ti ne-growth curves, and determining the values of IC50 and MFC. In addition, the potential mechanism of 10b against C. albicans strains was investigated by using thin-layer chromatography (TLC) assay and transmission electron microscope. RESULTS: 10b had stronger in vitro activity than FLC against C. albicans. The values of IC50 and MFC of 10b were 5.41 and 64 μg·mL-1 while the latter were 337.18 μg·mL-1 and ≤ 256 μg·mL-1, respectively. 10b markedly reduced ergosterol content in C. albicans but did not affect the activity of lanosterol 14a-demethylase (CYP51) and the integrality of cell membrane structure. CONCLUSION: 10b deserves further exploration and investigation for its potent antifungal activity and novel chemical structure.
