Application study of ITS/ITS2 barcodes in identification of Zanthoxmli Pericarpium
- Author:
Dian-Yun HOU
1
Author Information
- Publication Type:Journal Article
- Keywords: DNA barcode; ITS/ITS2; Molecular identification; Zanthoxmli Pericarpium
- From: Chinese Pharmaceutical Journal 2014;49(7):534-538
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To evaluate the accuracy and stability of ITS/ITS2 barcodes in identification of Zanthoxmli Pericarpium. METHODS: Total genomic DNAs from samples were extracted by using improved DNA extraction kits. ITS regions were amplified, and the ITS2 sequences were obtained by using the hidden Markov model (HMM) based on annotation method from the ITS sequences. The inter- and intra-specific variation of the Zanthoxmli Pericarpium and its adulterants were analyzed. Zanthoxmli Pericarpium was i-dentified through the Species identification System for Traditional Chinese Medicine and neighbor-joining (NJ) phylogenetic trees. RESULTS: The lengths of ITS/ITS2 sequence of Zanthoxmli Pericarpium were 618-620 bp and 224 bp. The intra-specific genetic distances were smaller than inter-specific ones in ITS/ITS2 regions of Z. schinifolium and Z. bungeanum. Zanthoxmli Pericarpium and its adulterants could be easily differentiated according to the nearest distance, the Species Identification System for Traditional Chinese Medicine and the NJ trees methods. CONCLUSION: ITS/ITS2 regions as DNA barcodes can stably and accurately distinguish Zanthoxmli Pericarpium and its adulterants.
