Simultaneous determination of six compounds in Niuhuang Shangqing pills by HPLC
- Author:
Cheng-Yi HAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Baiealin; Berberine hydrochloride; Chlorogenic acid; Ehrysophanol; Emodin; Geniposide; HPLC; Niuhuang Shangqing pills
- From: Chinese Pharmaceutical Journal 2014;49(9):769-772
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish a method for simultaneous determination of chlorogenic acid, geniposide, berberine, baiealin, emodin and ehrysophanol in Niuhuang Shangqing pills (traditional Chinese medicines). METHODS: The separation was performed on a DIKMA Platisil ODS column (4.6 mm×5 μm) with gradient elution using methanol and 0.1% H3PO4 aqueous solution as mobile phase at the flow rate of 1 mL·min-1. The UV detection wavelength was set at 254 nm. RESULTS: Linear ranges of the calibration curves of chlorogenic acid, geniposide, berberine, baiealin, emodin and ehrysophanol were 0.11-2.2 μg (r=0.9996), 0.048-0.96 μg (r=0.9998), 0.073-1.46 μg (r=0.999 9), 0.1-2 μg (r=0.9999), 0.04-0.8 μg (r=0.9995), and 0.01-0.2 μg (r=0.9996), respectively. Their average recoveries fell between 98.26% and 99.44%. CONCLUSION: The established HPLC method can be used to determine the contents of chlorogenic acid, geniposide, berberine, baiealin, emodin and ehrysophanol in Niuhuang Shangqing pills. The method can also be used for the quality control of Niuhuang Shangqing pills.
