Simultaneous quantitative analysis of four Triterpenoids in Alismatis Rhizoma by quantitative analysis of multi-components by single marker
- Author:
Wan-Li ZHAO
1
Author Information
- Publication Type:Journal Article
- Keywords: Alismatis Rhizoma; QAMS; Quality control; Triterpenoids
- From: Chinese Pharmaceutical Journal 2015;50(15):1347-1352
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To develop a simultaneous quantitative analysis method of four triterpenoids in Alismatis Rhizoma by multi-components by single maker (QAMS). METHODS: Four main triterpenoids (alisol A, alisol A 24-acetate, alisol B and alisol B 23-acetate) were selected as analytes to evaluate the quality of Alismatis Rhizoma. Alisol B 23-acetate was used as the internal reference standard and three relative correction factors (RCFs) to alisol B 23-acetate were calculated. These RCFs were obtained by high performance liquid chromatography coupled with diode array detector under various chromatographic conditions. Then 31 batches of Alismatis Rhizoma samples collected from different areas were determined by both external standard method (ESM) and QAMS. RESULTS: The RCFs were obtained with good reproducibility (RSD≤2.25%). No obvious differences were observed between the analysis results of QAMS method and ESM method (RSD≤3.52%). CONCLUSION: The established QAMS method is reliable and feasible for simultaneous analysis of four triterpenoids and could be used for quality control of Alismatis Rhizoma.
