Simultaneous determination of three nucleosides in Cordyceps sinensis by QAMS
- Author:
Zheng-Ming QIAN
1
Author Information
- Publication Type:Journal Article
- Keywords: Cordyceps sinensis; HPLC; Nucleoside; Quantitative analysis of multi-components by single-marker (QAMS); Relative correction factors (RCF)
- From: Chinese Pharmaceutical Journal 2015;50(15):1297-1300
- CountryChina
- Language:Chinese
- Abstract: OBJECTIVE: To establish a quantitative analysis of multi-components by single-marker (QAMS) method for determination of uridine, guanosine and adenosine in Cordyceps sinensis. METHODS: Adenosine was used as the marker to calculate the relative correction factors (RCF) for uridine and guanosine by using high performance liquid chromatography(HPLC). Both QAMS method and external standard method (ESM) were performed to determine the three kinds of nucleosides in Cordyceps sinensis. RESULTS: There were no significant differences between the two methods. CONCLUSION: The developed QAMS method is feasible to evaluate the quality of Cordyceps sinensis.
